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Clinical ResearchResearch compoundModerate evidence

Pregnanolone

Endogenous neurosteroid and GABA-A positive allosteric modulator, also developed as the anesthetic eltanolone.

NeurosteroidAnxiolyticAnxiolyticSleep SupportGABAnoo.affects.sleepnoo.affects.anxiety

Pharmacology

ClassAnxiolytic · Sleep Support
Primary targetGABA-A receptor positive allosteric modulation (neurosteroid)
Targets3 receptor targets
Half-lifeIntravenous eltanolone: short, with rapid clearance (distribution on the order of minutes); endogenous levels fluctuate quickly
OnsetEvening or before rest; calming and potentially sedating (within seconds when given intravenously as an anesthetic)
EvidenceModerate evidence
Affected systemsGABAnoo.affects.sleepnoo.affects.anxiety

Contents

WHAT IS PREGNANOLONE?

Detailed overview

Pregnanolone (eltanolone, 3alpha-hydroxy-5beta-pregnan-20-one) is an endogenous neurosteroid produced from progesterone by enzymatic conversion and closely related to allopregnanolone. Acting at a neurosteroid site on the GABA-A receptor, it works as a positive allosteric modulator that strengthens GABA's inhibitory effect, letting more chloride into neurons, lowering excitability and producing sedative, anti-anxiety and anticonvulsant effects. In the 1990s it was developed as the intravenous anesthetic eltanolone and tested in human clinical trials, but it was never marketed. Oral bioavailability is poor and it is rapidly metabolized, so it is not available as a practical supplement.

Mechanism

GABA-A positive allosteric modulator (neurosteroid)

Evidence

Human anesthesia trials (eltanolone)

Legal status

Not marketed (was a Phase III anesthetic)

Receptor profile

  • GABA-A receptorsStrong
  • Anxiety and stress modulationModerate
  • NMDA receptors (in sulfated form)Weak

Safety

Side effects, stop signs, contraindications

Side effects · 6

  • Sedation and drowsiness
  • Respiratory depression (slowed breathing) at anesthetic doses
  • Changes in heart rate and blood pressure
  • Involuntary movements, rarely convulsions at high doses
  • Local injection-site reaction or pain (intravenous eltanolone lipid emulsion)
  • Essentially inactive orally due to poor oral bioavailability

Contraindications · 4

  • Pregnancy and breastfeeding: safety not established, avoid
  • With other CNS depressants (alcohol, benzodiazepines, opioids): dangerous additive sedation and respiratory depression
  • Respiratory insufficiency or severe hepatic disease
  • Avoid driving and operating machinery due to the sedative effect

Related Nootropics

Same therapeutic category

Studies

Related research and clinical findings

FAQ

FAQ

Endogenous neurosteroid and GABA-A positive allosteric modulator, also developed as the anesthetic eltanolone.

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Structure & chemistry

TypeNeurosteroid
FormulaC21H34O2
UpdatedJuly 10, 2026
MolekulaX Editorial Team·Source-verified · PubMed · FDA · EMA
Updated: July 10, 2026

The information here is strictly for educational and scientific purposes. It does not replace medical advice or clinical consultation, and it does not encourage illegal substance or pharmaceutical use. Data is sourced. When in doubt, consult your doctor.